Coding

Part:BBa_K4644024:Design

Designed by: Han Cao   Group: iGEM23_BUCT   (2023-10-10)


DnaK


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 727
    Illegal PstI site found at 860
    Illegal PstI site found at 970
    Illegal PstI site found at 1321
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 727
    Illegal PstI site found at 860
    Illegal PstI site found at 970
    Illegal PstI site found at 1321
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 727
    Illegal BglII site found at 930
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 727
    Illegal PstI site found at 860
    Illegal PstI site found at 970
    Illegal PstI site found at 1321
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 727
    Illegal PstI site found at 860
    Illegal PstI site found at 970
    Illegal PstI site found at 1321
    Illegal NgoMIV site found at 156
    Illegal NgoMIV site found at 1671
    Illegal AgeI site found at 399
    Illegal AgeI site found at 871
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We constructed pcs-lac-DnaKJ plasmid

Source

E.coli

References

Judith Matavacas, Joel Hallgren, Claes von Wachenfeldt, Bacillus subtilis forms twisted cells with cell wall integrity defects upon removal of the molecular chaperones DnaK and trigger factor, Frontiers in Microbiology, 13, (2023).

Gamer, J., Multhaup, G., Tomoyasu, T., McCarty, J.S., Rüdiger, S., Schönfeld, H.J., Schirra, C., Bujard, H. and Bukau, B. (1996), A cycle of binding and release of the DnaK, DnaJ and GrpE chaperones regulates activity of the Escherichia coli heat shock transcription factor sigma32.. The EMBO Journal, 15: 607-617.